Allelic series six month dataset
The linked Excel workbook below contains the full FPKM and count data, along with their associated sample annotations for the 6 month allelic series mRNA sequencing performed by Expression Analysis at the behest of CHDI. The data consists of samples from the striatum, cortex and liver of knock in mice with CAG lengths of 20, 80, 92, 111, 140, 175 along with littermate control wildtype animals. There are four animals per sex per CAG length totaling 188 samples (4 samples were removed for QC reasons). Pair-end sequencing was performed to a depth of 40 million reads. Alignment and mapping was performed using Omicsoft’s OSA algorithm against mouse reference genome B37 and genes mapping performed using ENSEMBL release 67. For additional information on the quality control procedures see the PowerPoint slide deck linked below.